fluorescent sphingosine analog Search Results


synthetic fluorescent phospholipid analogue  (Avanti Polar)
96
Avanti Polar synthetic fluorescent phospholipid analogue
Synthetic Fluorescent Phospholipid Analogue, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+sphingosine+analog/C24+Mono-Sulfo+Galactosyl(%C3%9F)+Ceramide/pmc08657686-207-13-19
Average 96 stars, based on 1 article reviews
synthetic fluorescent phospholipid analogue - by Bioz Stars, 2026-10
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fluorescent sphingosine analog  (Croda International Plc)
98
Croda International Plc fluorescent sphingosine analog
Fluorescent Sphingosine Analog, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+sphingosine+analog/Sphingosine/pm41860929-120-1-11
Average 98 stars, based on 1 article reviews
fluorescent sphingosine analog - by Bioz Stars, 2026-10
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fluorescent ceramide analog nbd c6 ceramide  (Avanti Polar)
95
Avanti Polar fluorescent ceramide analog nbd c6 ceramide
A model for the role of Inositol-phosphoryl <t>ceramide</t> synthase 1 (Ipc1) in regulation of virulence traits and pathogenesis of C. neoformans. Ipc1 transfers the phosphoryl-inositol moiety from phosphatidylinositol (PI) to phytoceramide, forming inositol-phosphoryl ceramide (IPC) and diacylglycerol (DAG).
Fluorescent Ceramide Analog Nbd C6 Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+sphingosine+analog/C6+Ceramide/pmc00312614-308-12-16
Average 95 stars, based on 1 article reviews
fluorescent ceramide analog nbd c6 ceramide - by Bioz Stars, 2026-10
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A model for the role of Inositol-phosphoryl ceramide synthase 1 (Ipc1) in regulation of virulence traits and pathogenesis of C. neoformans. Ipc1 transfers the phosphoryl-inositol moiety from phosphatidylinositol (PI) to phytoceramide, forming inositol-phosphoryl ceramide (IPC) and diacylglycerol (DAG).

Journal:

Article Title: Roles for inositol-phosphoryl ceramide synthase 1 ( IPC1 ) in pathogenesis of C. neoformans

doi:

Figure Lengend Snippet: A model for the role of Inositol-phosphoryl ceramide synthase 1 (Ipc1) in regulation of virulence traits and pathogenesis of C. neoformans. Ipc1 transfers the phosphoryl-inositol moiety from phosphatidylinositol (PI) to phytoceramide, forming inositol-phosphoryl ceramide (IPC) and diacylglycerol (DAG).

Article Snippet: In vitro activity of Ipc1 Ipc1 activity was measured by using the fluorescent ceramide analog NBD-C6-ceramide (Avanti Polar Lipids) as substrate and monitoring the formation of NBC-C6-IPC, as described by Fischl et al. (2000) with some modifications.

Techniques:

(A) In vitro quantitative analysis of C. neoformans IPC1 mRNA in H99 and GAL7::IPC1 strains. The isogenic wild-type strain H99 (WT) and the GAL7::IPC1 strains were grown on galactose (inducing conditions). RNA was extracted at different time points (30 min; 1, 3, 6, and 24 h), converted to cDNA, and amplified with primers directed to IPC1 or actin genes. RT-PCR products were separated by electrophoresis in a 1% agarose gel and stained with ethidium bromide. The IPC1 and actin RT-PCR products are 926 and 543 bp, as indicated. (B) In vitro quantitative analysis of Ipc1 activity in C. neoformans wild-type (H99) and GAL7::IPC1 strains grown on glucose (repressing conditions) and galactose (inducing conditions). (Left) The Ipc1 synthase in vitro activity was determined as conversion of NBD-C6-ceramide (NBD-C6-CER) into NBC-C6-IPC by thin layer chromatography (TLC) analysis. (Right) Numerical analysis of Ipc1 activity from the TLC is reported.

Journal:

Article Title: Roles for inositol-phosphoryl ceramide synthase 1 ( IPC1 ) in pathogenesis of C. neoformans

doi:

Figure Lengend Snippet: (A) In vitro quantitative analysis of C. neoformans IPC1 mRNA in H99 and GAL7::IPC1 strains. The isogenic wild-type strain H99 (WT) and the GAL7::IPC1 strains were grown on galactose (inducing conditions). RNA was extracted at different time points (30 min; 1, 3, 6, and 24 h), converted to cDNA, and amplified with primers directed to IPC1 or actin genes. RT-PCR products were separated by electrophoresis in a 1% agarose gel and stained with ethidium bromide. The IPC1 and actin RT-PCR products are 926 and 543 bp, as indicated. (B) In vitro quantitative analysis of Ipc1 activity in C. neoformans wild-type (H99) and GAL7::IPC1 strains grown on glucose (repressing conditions) and galactose (inducing conditions). (Left) The Ipc1 synthase in vitro activity was determined as conversion of NBD-C6-ceramide (NBD-C6-CER) into NBC-C6-IPC by thin layer chromatography (TLC) analysis. (Right) Numerical analysis of Ipc1 activity from the TLC is reported.

Article Snippet: In vitro activity of Ipc1 Ipc1 activity was measured by using the fluorescent ceramide analog NBD-C6-ceramide (Avanti Polar Lipids) as substrate and monitoring the formation of NBC-C6-IPC, as described by Fischl et al. (2000) with some modifications.

Techniques: In Vitro, Amplification, Reverse Transcription Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Staining, Activity Assay, Thin Layer Chromatography